文章摘要
陈素辉,孙华,徐虹,张亚敏,王富明,高扬.针刺百会和足三里穴对脑缺血再灌注损伤大鼠双侧脑组织白细胞介素-6表达的影响[J].针灸临床杂志,2014,3(1):42-45,F0003
针刺百会和足三里穴对脑缺血再灌注损伤大鼠双侧脑组织白细胞介素-6表达的影响
Effects of Acupuncture of“Baihui”(GV20) and“Zusanli”(ST36) on the Expression of Interleukin-6 in Bilateral Rats'Brain after Cerebral Ischemia Reperfusion Injury
  
DOI:
中文关键词: 针刺  百会  足三里  脑缺血再灌注损伤  白细胞介素-6
英文关键词: Acupuncture  Cerebral ischemia reperfusion injury
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陈素辉,孙华,徐虹,张亚敏,王富明,高扬  
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中文摘要:
      目的:探讨针刺百会和足三里穴对脑缺血再灌注损伤大鼠双侧脑组织白细胞介素-6的表达变化。方法:采用随机数字表将SD大鼠随机分为针刺组(A)、模型组(M)和假手术组(S)3个大组,各大组再根据大鼠再灌注后时间分为12 h、24 h、48 h、72 h、96 h和144 h共6个时间组,每个时间组有6只大鼠,设正常组( N)6只。模型组及针刺组应用线栓法制备脑缺血再灌注损伤大鼠模型,模型组造模成功后不做治疗干预;针刺组造模成功后,取百会和足三里穴进行针刺治疗20 min,每天1次;假手术组未插入线栓,其余同模型组;正常组未进行处理及治疗。应用免疫组化法检测大鼠双侧脑组织IL-6的表达。结果:IL-6在脑缺血大鼠患侧脑区呈单峰表达,模型组峰值时间为96 h,针刺组为72 h,针刺组表达量显著低于模型组,但高于假手术组和正常组;IL-6在脑缺血大鼠健侧脑区表达高于假手术组和正常组,除12 h和96 h组外,针刺组各个时间点的表达高于模型组( P<0.05)。结论:针刺百会和足三里穴可通过调节脑缺血损伤大鼠双侧脑组织IL-6的表达,干预脑缺血再灌注损伤大鼠的炎症反应。
英文摘要:
      Objective:To explore the effects of acupuncture of DV 20 and ST36 on the expression of interleukin-6 in rats'brain after cerebral ischemia reperfusion injury ( CIRI) .Methods:CIRI rat models were established by right middle cerebral artery occlusion and reperfusion .SD rats were randomly divided into four groups:acu-puncture group (A), model group (M), sham-operation group (S) and normal control group (N).The Group M didn't do any disposal after successful modeling .The Group A was acupunctured at Baihui ( GV20 ) and Zusanli ( ST36) for 20 min once a day after successful operation .The Group S was to imitate the operation procedure , without inserting line bolt into artery and with no treatment .The Group N was regarded as blank control group , without any operation and treatment .The expression of Interleukin -6 ( IL-6) in bilateral brain tissues was examined by immunohistochemistry .Results:The expression of IL -6 in cerebral ischemia regions exhibited a single-peak, the peak time in CIRI of group M was at 96h, while in group A it was at 72h, com-pared with group M , the expression of IL -6 in group A declined obviously , but the level was still above the group S and group N; the expression of IL -6 in cerebral non -ischemia regions was above the group S and group N, except the time-points of 12h and 96h,and the expression in group A was higher than that in group M.Conclusion:Acupuncture of GV20 and ST36 can affect the expression of IL -6 in bilateral cerebral tissues of CIRI rats, interfering the inflammation response of CIRI .
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