文章摘要
针刺对脑卒中模型大鼠认知功能障碍影响及其与PI3K/Akt信号转导通路关系
Effect of acupuncture on cognitive dysfunction in rats with stroke model and its relationship with PI3K/Akt signal transduction pathway
投稿时间:2019-06-13  录用日期:2019-06-25
DOI:
中文关键词: 脑卒中  认知功能障碍  PI3K/Akt信号通路  针刺
英文关键词: stroke  cognitive dysfunction  PI3K/Akt signaling pathway  acupuncture
基金项目:湖北省自然科学基金计划项目 (项目编号:2011CDC146)
作者单位E-mail
伍芳 恩施土家族苗族自治州中心医院 d16666@sina.com 
胡振平 恩施土家族苗族自治州中心医院  
谢远见 恩施土家族苗族自治州中心医院  
廖家权 恩施土家族苗族自治州中心医院  
唐昌敏 恩施土家族苗族自治州中心医院  
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中文摘要:
      目的:分析针刺对脑卒中模型大鼠认知功能障碍影响及其与磷酸酰肌醇-3-激酶(PI3K)/蛋白激酶B(Akt)信号转导通路联系。方法:选取SPF级雄性大鼠30只,随机数字表法将大鼠分成3组,分别为针刺组、模型组和正常组,针刺组、模型组大鼠制备大脑中动脉梗塞(MCAO)模型,成功造模后电针仪对针刺组大鼠行针刺治疗,模型组与正常组不进行任何处理。观察大鼠神经功能评分、脑梗死面积、脑组织细胞凋亡率、脑组织PI3K、Bcl-2、p-Akt、t-Akt、Bax蛋白表达及Bax、Bcl-2及Bc1-2/Bax mRNA表达。结果:和正常组相比,模型组大鼠脑梗死面积增大;和模型组相比,针刺组大鼠脑梗死面积减少,差异有统计学意义(P<0.05);和正常组相比,模型组大鼠凋亡阳性细胞比例升高;和模型组相比,针刺组大鼠凋亡阳性细胞比例降低,差异均有统计学意义(P<0.05);和正常组相比,模型组大鼠Bax蛋白表达升高,PI3K、Bcl-2、p-Akt蛋白表达降低;和模型组相比,针刺组大鼠Bax蛋白表达降低,PI3K、Bcl-2、p-Akt蛋白表达升高,差异有统计学意义(P<0.05);和正常组相比,模型组大鼠Bax mRNA表达升高,Bcl-2及Bc1-2/Bax mRNA表达降低;和模型组相比,针刺组大鼠Bax mRNA表达降低,Bcl-2及Bc1-2/Bax mRNA表达升高,差异有统计学意义(P<0.05)。结论:针刺脑卒中大鼠可显著改善其认知功能障碍,加速神经功能恢复,作用机制可能将PI3K/Akt路径激活,对神经细胞凋亡抑制有联系。
英文摘要:
      Objective:To analyze the effects of acupuncture on cognitive dysfunction in rats with stroke and its association with phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway.Methods:Thirty male SPF rats were randomly divided into three groups: acupuncture group, model group and normal group. Acupuncture group and model group were used to prepare middle cerebral artery infarction (MCAO) model. After successful modeling, the electroacupuncture instrument was treated with acupuncture in the acupuncture group, and the model group and the normal group were not treated. The neurological function score, cerebral infarction area, brain tissue apoptosis rate, brain tissue PI3K, Bcl-2, p-Akt, t-Akt, Bax protein expression and Bax, Bcl-2 and Bcl-2/Bax mRNA were observed. expression.Results:Compared with the normal group, the infarct size of the model group increased. Compared with the model group, the infarct size of the rats in the acupuncture group decreased, the difference was statistically significant (P<0.05); compared with the normal group. The proportion of apoptosis-positive cells in the model group was increased. Compared with the model group, the percentage of apoptosis-positive cells in the acupuncture group was decreased, and the difference was statistically significant (P<0.05). Compared with the normal group, the model The expression of Bax protein was increased and the expression of PI3K, Bcl-2 and p-Akt protein was decreased in the rats. Compared with the model group, the expression of Bax protein was decreased and the expression of PI3K, Bcl-2 and p-Akt protein was increased in the acupuncture group. The difference was statistically significant (P<0.05). Compared with the normal group, the expression of Bax mRNA was increased and the expression of Bcl-2 and Bcl-2/Bax mRNA was decreased in the model group. Compared with the model group, acupuncture was performed. The expression of Bax mRNA was decreased and the expression of Bcl-2 and Bcl-2/Bax mRNA was increased in the rats. The difference was statistically significant (P<0.05).Conclusion:Acupuncture stroke rats can significantly improve their cognitive dysfunction and accelerate the recovery of neurological function. The mechanism may activate PI3K/Akt pathway and correlate with inhibition of neuronal apoptosis.
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