| Objective:To analyze the effects of acupuncture on cognitive dysfunction in rats with stroke and its association with phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) signal transduction pathway.Methods:Thirty male SPF rats were randomly divided into three groups: acupuncture group, model group and normal group. Acupuncture group and model group were used to prepare middle cerebral artery infarction (MCAO) model. After successful modeling, the electroacupuncture instrument was treated with acupuncture in the acupuncture group, and the model group and the normal group were not treated. The neurological function score, cerebral infarction area, brain tissue apoptosis rate, brain tissue PI3K, Bcl-2, p-Akt, t-Akt, Bax protein expression and Bax, Bcl-2 and Bcl-2/Bax mRNA were observed. expression.Results:Compared with the normal group, the infarct size of the model group increased. Compared with the model group, the infarct size of the rats in the acupuncture group decreased, the difference was statistically significant (P<0.05); compared with the normal group. The proportion of apoptosis-positive cells in the model group was increased. Compared with the model group, the percentage of apoptosis-positive cells in the acupuncture group was decreased, and the difference was statistically significant (P<0.05). Compared with the normal group, the model The expression of Bax protein was increased and the expression of PI3K, Bcl-2 and p-Akt protein was decreased in the rats. Compared with the model group, the expression of Bax protein was decreased and the expression of PI3K, Bcl-2 and p-Akt protein was increased in the acupuncture group. The difference was statistically significant (P<0.05). Compared with the normal group, the expression of Bax mRNA was increased and the expression of Bcl-2 and Bcl-2/Bax mRNA was decreased in the model group. Compared with the model group, acupuncture was performed. The expression of Bax mRNA was decreased and the expression of Bcl-2 and Bcl-2/Bax mRNA was increased in the rats. The difference was statistically significant (P<0.05).Conclusion:Acupuncture stroke rats can significantly improve their cognitive dysfunction and accelerate the recovery of neurological function. The mechanism may activate PI3K/Akt pathway and correlate with inhibition of neuronal apoptosis. |
