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| 以电针对照观察针刀干预对膝骨关节炎兔股直肌的修复作用 |
| Using electroacupuncture as a control to observe the effect of Acupotomy intervention on repair of rectus femoris muscle in rabbits with knee osteoarthritis |
| 投稿时间：2019-07-02 录用日期：2019-07-09 |
| DOI： |
| 中文关键词: 针刀 股直肌萎缩 膝骨关节炎 肌肉修复 |
| 英文关键词: Acupotomy Atrophic rectus knee osteoarthritis Muscle repair |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 目的：探讨针刀对于膝骨关节炎（KOA）兔股直肌萎缩的修复作用。方法：雄兔随机分为针刀组（A）、电针组（EA）、正常组（N）和模型组（M），各9只。采用左后肢膝关节制动6周制备退行性膝骨关节炎模型。N组与M组不予干预，A组和EA组各干预4周。采用Western Blot法观测兔股直肌中Akt、mTOR、p70s6k的蛋白含量。结果：①M组较N组股直肌中mTOR和p70s6k、Akt蛋白的表达水平明显下降（P<0.01或P<0.05）；②股直肌Akt蛋白表达：A组与EA组相比M组显著升高（P＜0.01）；③股直肌mTOR蛋白表达：相比M组，A组与EA组升高（P＜0.01或P＜0.05）；④股直肌p70s6k蛋白表达：A组较M组显著升高（P＜0.05），而EA组与M组无显著差异（P＞0.05）。结论：针刀通过修复萎缩的股直肌，改善膝周软组织的生物力学，促进膝关节的恢复。 |
| 英文摘要: |
| Objective: To investigate the repairing effect of Acupotomy on atrophy of rabbit rectus muscle in knee osteoarthritis (KOA). Methods: Male rabbits were randomly divided into acupuncture group (A), electroacupuncture group (EA), normal group (N) and model group (M), with 9 rabbits in each group. The knee osteoarthritis model was prepared by braking the left hind limb knee joint for 6 weeks. group N and group M were not intervened but group A and group EA were interfered for 4 weeks. The protein expression of Akt, mTOR and p70s6k in the rectus femoris muscle was detected by Western Blot. Results:① The expression levels of mTOR, p70s6k and Akt in the rectus femoris of group M were significantly lower than those in group N (P<0.01 or P<0.05). ②The expression of Akt protein in rectus muscles : group A and group EA was significantly higher than those in group M (P<0.01);③mTOR protein expression in rectus muscle: compared with group M, group A and EA group increased (P<0.01 or P<0.05); ④p70s6k protein expression in rectus muscle: There was a significant increase in the group A compared with group M (P<0.05), but there was no significant difference between the EA group and the M group (P>0.05). Conclusion: Acupotomy can promote the recovery of knee joint by repairing rectus femoris and improving the biomechanics of knee peri-soft tissue. |
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