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电针对2型糖尿病ZDF大鼠骨骼肌PI3K p85和GLUT4的表达影响 |
Effect of Electroacupuncture on the expression of PI3K p85 and GLUT4 in skeletal muscle of ZDF rats with type 2 diabetes mellitus |
投稿时间:2019-09-01 录用日期:2019-09-30 |
DOI: |
中文关键词: 电针 2型糖尿病 磷脂酰肌醇 3 激酶 葡萄糖转运蛋白 4 |
英文关键词: electroacupuncture type 2 diabetes mellitus phosphatidylinositol 3 kinase glucose transporter 4 |
基金项目:国家自然科学基金项目(青年) |
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中文摘要: |
摘要:目的:观察电针对2型糖尿病ZDF大鼠血糖、PI3K p85及GLUT4蛋白表达影响,方法:选取14只体质量140-160g(2月龄)SPF级雄性Zucker Diabetic Fatty,ZDF(fa/fa)大鼠,随机分为模型组、电针组;另取7只同月龄SPF级雄性Zucker Lean,ZL(fa/+)大鼠作为对照组。连续干预4周,测定各组空腹血糖。4周后Western blot检测骨骼肌的磷脂酰肌醇3激酶(PI3K)和葡萄糖转运蛋白4(GLUT4)蛋白表达水平。结果:干预后与对照组比较,模型组空腹血糖显著升高(P<0.01),与模型组比较,电针组空腹血糖显著下降(P<0.05)。Western bolt与对照组对比,模型组PI3K p85,GLUT4蛋白表达量显著下降(P<0.01),与模型组比较,电针组PI3K p85,GLUT4蛋白表达显著升高(P<0.01)。结论:电针干预可改善ZDF大鼠空腹血糖及骨骼肌细胞的IR。其机制为可能通过PI3K-GLUT4信号传导途径有关。 |
英文摘要: |
ABSTRACT: AIM: To observe the effects of Electroacupuncture on blood sugar, PI3K p85 and GLUT4 protein expression in ZDF rats with type 2 diabetes mellitus. METHODS: Fourteen SPF male Zucker Diabetic Fatty and ZDF (fa/fa) rats weighing 140-160g were randomly divided into model group and electro-acupuncture group, and another seven SPF male Zucker Lean, ZL (fa/fa) rats of the same age were selected./ +) Rats were taken as control group. After 4 weeks of intervention, fasting blood glucose was measured in each group. Four weeks later, Western blot was used to detect the expression of phosphatidylinositol 3 kinase (PI3K) and glucose transporter 4 (GLUT4) in skeletal muscle. Results: Compared with the control group, the fasting blood sugar in the model group increased significantly (P < 0.01), and decreased significantly (P < 0.05) compared with the model group. Compared with the control group, the expression of PI3K p85 and GLUT4 protein in the model group decreased significantly (P < 0.01). Compared with the model group, the expression of PI3K p85 and GLUT4 protein in the EA group increased significantly (P < 0.01). CONCLUSION: Electroacupuncture can improve fasting blood sugar and IR of skeletal muscle cells in ZDF rats. The mechanism may be related to PI3K-GLUT4 signal pathway. |
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