| This study adopts a bioinformatics/network topology strategy to explore the targets and molecular mechanisms of acupuncture treatment for constipation. Method: Using literature search combined with bioinformatics methods, we searched for effective substances of acupuncture intervention in constipation, and searched for effective targets of constipation through databases such as Genecards. Import the effector substances into the STITCH and Swiss Target Prediction databases to obtain regulatory targets. Intersect the disease and the target effects of acupuncture, obtain potential targets for acupuncture intervention in constipation, construct a PPI interaction network, and draw a regulatory diagram for further GO/KEGG analysis. After obtaining the above targets, use Cytoscape topology analysis method to screen the core target of action.Result: Through literature review and acupuncture network research, we found that the active compound of acupuncture intervention in constipation may be 5-TH, which may regulate core targets such as SLC6A4, MAOA, SLC6A3, SLC6A2, and MAOB, activate signaling pathways such as Calcium, cGMP PKG, Rap1, cAMP, and Ras, mediate G protein coupled amines, receptor activity, and neurotransmitter receptor activity pathological reactions, thereby exerting a therapeutic effect on constipation. Conclusion: Acupuncture treatment for constipation is highly likely to be effective by generating 5-TH, activating signaling pathways such as Calcium, cGMP PKG, Rap1, cAMP, Ras, and regulating core targets such as SLC6A4, MAOA, SLC6A3, SLC6A2, and MAOB. |
